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Image Search Results
Journal: Human Molecular Genetics
Article Title: PD-linked CHCHD2 mutations impair CHCHD10 and MICOS complex leading to mitochondria dysfunction
doi: 10.1093/hmg/ddy413
Figure Lengend Snippet: PD-linked CHCHD2 mutants showed reduced binding to CHCHD10. ( A ) Co-immunoprecipitation of endogenous CHCHD2 by antibody against CHCHD2 in SK-N-SH cells. WCL: whole cell lysate, 5% total protein used in co-IP experiment. ( B ) Co-immunoprecipitation of endogenous CHCHD10 by antibody against CHCHD10 in SK-N-SH cells. WCL: whole cell lysate, 5% total protein used in co-IP experiment. ( C ) Co-immunoprecipitation of endogenous CHCHD2 by antibody against CHCHD2 in human brain tissue lysates. ( D ) Co-immunoprecipitation of CHCHD2 by a CHCHD2 antibody against middle region of CHCHD2 (labeled as CHCHD2-TF) in SK-N-SH cells transfected with non-tagged CHCHD2 WT/T61I/R145Q/Q126X. WCL: whole cell lysate, 5% total protein used in co-IP experiment. Lower arrow pointed to CHCHD2 Q126X and higher arrow pointed to full length CHCHD2. ( E ) Co-immunoprecipitation of CHCHD2 by a CHCHD2 antibody against middle region of CHCHD2 (labeled as CHCHD2-TF) in hESCs of H9 and isogenic lines harboring homozygous R145Q (−/−).WCL: whole cell lysate, 5% total protein used in co-IP experiment. Arrow pointed to CHCHD10. Representative results from R10 and R17 (R145Q−/−) were shown. ( F ) Quantification of protein abundance of CHCHD10 normalized with CHCHD2 on the co-immunoprecipitation complex from isogenic hESC lysates.
Article Snippet:
Techniques: Binding Assay, Immunoprecipitation, Co-Immunoprecipitation Assay, Labeling, Transfection, Quantitative Proteomics
Journal: bioRxiv
Article Title: Design of M protein immunogens to elicit broadly reactive antibodies against Streptococcus pyogenes
doi: 10.1101/2021.12.07.471543
Figure Lengend Snippet: Reactivity of M2G and M2 antisera against normal adult human brain tissue lysate (HB) or heart tissue lysate (HH), as determined by western blot analysis. Intact M2 protein (M2) was used as a positive control. Each blot is representative of three experimental replicates. a, M2G antiserum (Ab(M2G)). b, M2 antiserum (Ab(M2)). c, Input samples visualized by Coomassie-stained SDS-PAGE.
Article Snippet: Twenty μg of normal
Techniques: Western Blot, Positive Control, Staining, SDS Page